answer the question

Question 1
Which organisms would you add to a commercial inoculant mix for controlling banana’s root disease and for enhancing the growth of banana seedlings? Select from the list below microbial agents you would include in the inoculat mix.
Select more than one.
a. Ethylene producing bacteria
b. Phosphate solubilising Actinomycetes
c. Hormone producing fungi
d. Trichoderma that produces a wide spectrum of antibacterial
e. Nitrogen fixing Azospirillum f. Trichoderma that is not antagonistic to soil bacteria
THE COORECT ANSWER IS :

Question 2
Suggest which of the procedures below could be used for producing a nitrogen-fixing organism that could be used as inoculant in desert soils. More than one procedure could be used.
Select one or more:
a. Use an extremely efficient nitrogen fixing bacterium which requires high moisture for its survival
b. Use a drought tolerant bacterium to create a recombinant strain that has the nif genes encoding nitrogen fixation inserted into the genome
c. Clone the drought genes into a bacterium that can already obtain nitrogen from its environment
d. Isolate a nitrogen fixing bacterium from the Sahara desert
THE COORECT ANSWER IS :

Question 3
Which of the listed consideration/s need/s to be taken into account for the use and commercialisation of plant growth promoting microbes for crop production?
Select one or more:
a. The effects on other organisms in the environment
b. Shelf life of the inoculant product
c. The compatibility of inoculants in mixes
d. Soil type
e. The composition of indigenous microbial communities within soils

Question 4
Programmable nucleases do NOT need to:
Select one:
a. Recognise a specific sequence in double stranded RNA
b. Act at a pre-determined locus
c. Induce cleavage in the genome
d. Be programmed by researchers
Question 5
Which of the following statements describing zinc finger nucleases is most correct?
Select one:
a. Zinc finger DNA binding domains bind to a recognition site and cut double stranded DNA
b. Each zinc finger recognises a 3 base-pair sequence and binding domains are generally greater than 28 nucleotides long
c. ZFN binding is initiated by a spacer of 5-7 bp
d. FokI nuclease domains of ZFN monomers must dimerize to cleave DNA
Question 6
A PhD student researching cystic fibrosis wishes to use TALENs as part of a process to introduce transgenic material into a cell.
Which of the following steps would not occur in the process? Select one:
a. Integration of transgenic material by non-homologous repair
b. Delivery of developed TALENs to the cell nucleus
c. Selection of a target nucleotide sequence in the genome
d. Design of customised TALE repeat DNA-binding domains
Question 7
Each of the following steps are involved in the production of transgenic mice by pronuclear injection, EXCEPT for: Select one:
a. Vasectomy of male mice
b. Injection of DNA into the male pronucleus
c. Implantation of injected zygotes into the oviduct of a pseudopregnant foster mother
d. Isolation of zygotes from pregnant mice

Question 8
Which of the following statements regarding retroviral vectors is NOT true? Select one:
a. The host cell treats viral DNA as a part of its own DNA
b. Retroviral vectors infect the host cell and release the DNA genome into the cytoplasm
c. Reverse transcriptase produces a DNA copy of the genome
d. Viral DNA is inserted into the host cell with integrase
Question 9
Is it possible for a pig to be genetically modified to produce human proteins in the milk or semen of the animal? Select one:
a. No. A pig cannot be engineered to produce human proteins, however proteins naturally occurring in the pig may be modified
b. Yes, this is possible
c. It is possible for human proteins to be produced in pig milk, but not semen
d. No. DNA from a pig is too different from human DNA, however an animal with more similar homology may be engineered to produce human proteins
Question 10
Transgenic mice may be used to provide information about human diseases. A mutation in the human p53 gene is associated with an increased cancer risk. Which of the following methods would allow substitution of a healthy mouse p53 gene with a functional gene locus for a mutant human gene? Select one:
a. Transgenesis
b. Bioreaction
c. Gene Knockout
d. Gene Knock-in
Question 11
Which of the following is NOT a limitation to the use of genetically engineered animals in the production of human pharmaceuticals? Select one:
a. All of the optios provided are limitations
b. Incorporation of human genes into animals may have multiple deleterious pleiotropic effects
c. Genes may be overexpressed causing increased production of a product
d. There is a high risk of contamination from transfer of infectious diseases